The biceps tendon can be seen in longitudinal orientation by placing the back of the patient's hand on their ipsilateral thigh while seated, allowing 90 degree flexion of the elbow. The transducer is placed in sagittal position, allowing clear visualization of the tendon fibers in this orientation.

Fluid surrounding the biceps tendon within the tendon sheath is a fairly sensitive indicator of glenohumeral pathology, though as this space communicates with the shoulder capsule, such pathology is not necessarily limited to the biceps tendon and may represent a sign of synovitis or adhesive capsulitis.